Composite

Part:BBa_K5403020:Design

Designed by: Lizette van der Ziel   Group: iGEM24_TU-Eindhoven   (2024-10-02)


Fusion protein for FtsQ-based membrane anchoring of GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 41
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 41
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
    Illegal XhoI site found at 1441
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 41
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 41
    Illegal NgoMIV site found at 708
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 375
    Illegal BsaI.rc site found at 136
    Illegal SapI.rc site found at 203
    Illegal SapI.rc site found at 287


Design Notes

The sequence was codon-optimized for expression in M. smegmatis. Several modelling tools were used to aid in the design of this fusion protein (DeepTMHMM, Alphafold).


Source

M. smegmatis (FtsQ) and Aequorea victoria (GFP).

References